5. The Pain
That’s right, everyone’s favorite Cold War beekeeper starts off our list at number five. The Pain makes a few appearances before his fight, including having bees attack you during one of the Ocelot encounters that turns into you knifing bees out of the sky as Ocelot spins his revolvers to kill them. His fight is the most memorable moment, which starts out by summoning bee armor, throwing bee grenades, and attacking you with a bee tommy gun. Yes, a bee tommy gun.
As you lower his health throughout the fight he gets more aggressive before a cutscene triggers showing his final attack, spitting bees at you. And the best part is, they make jet noises as they fly towards you. The Pain goes out as memorably as he appears, however, as when you finally defeat him he explodes into an inferno of screams and bees.