Agitha
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To unlock Agitha, you must work your way through the main Adventure Mode map to square 4-7. That’s the fourth row down, seventh column in from the left. Before entering the challenge, you must use a Water Bomb item card to blow up the large rock in the water. Blowing up the rock unlocks Agitha as a new reward for completing that stage.
From there simply complete stage 4-7, and Agitha will be playable in all modes. Her starting weapon is the Butterfly Parasol.
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